Osazone reaction
and formation: -
Osazone is a class of carbohydrate derivative which is obtained when a sugar molecule is being treated with excess of phenylhydrazine. In the process of Osazone reaction and formation, the presence of a free carbonyl (aldehyde or keto) group is essential. A monosaccharide is being heated to 100 degree-celsius in the presence of phenylhydrazine. The acetic acid is changed to sugar phenylhydrazone in the reaction which then further reacts with the left-over reagent to form sugar Osazone. A precipitate is formed having a characteristic yellow or orange microscopic crystals having characteristic melting point.
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